Quantabio – Realtime qPCR Reagent
PerfeCTa qPCR Reagents combine a stringent, ultrapure antibody hotstart with performance engineered DNA polymerase in stabilized 1-tube formulations optimized for the specific performance needs of real-time quantitative PCR. Proprietary additives help eliminate persistent bubbles to enable efficient vortex mixing and fewer technical replicates thereby conserving precious sample. Adaptive buffer chemistry accommodates most assay designs and can be used with existing assay designs. PerfeCTa qPCR ToughMix reagents contain proprietary inhibitor-neutralizing additives that relieve several types of PCR inhibition ensuring reliable qPCR assay performance with clinical, plant or environmental specimens or complex food matix.
Available Models
| Product | Kit Size | Order Info |
|---|---|---|
| UltraPlex 1-Step ToughMix | 100 x 20 µL rxns (1 x 500 µL) | 95166-100 |
| 500 x 20 µL rxns (5 x 500 µL) | 95166-500 | |
| 1000 x 20 µL rxns (1 x 5 mL) | 95166-01K | |
| 10000 x 20 µL rxns (1 x 50 mL) | 95166-10K | |
| UltraPlex 1-Step ToughMix ROX | 100 x 20 µL rxns (1 x 500 µL) | 95167-100 |
| UltraPlex 1-Step ToughMix Low ROX | 500 x 20 µL rxns (5 x 500 µL) | 95168-500 |
| 100 x 20 µL rxns (1 x 500 µL) | 95168-100 | |
| 1000 x 20 µL rxns (1 x 5 mL) | 95168-01K | |
| 10000 x 20 µL rxns (1 x 50 mL) | 95168-10K |
UltraPlex 1-Step ToughMix is a ready-to-use, 4X concentrated master mix 1-step reverse transcription and real-time quantitative PCR (RT-qPCR) of RNA templates using probe-based detection methods. First-strand cDNA synthesis and PCR amplification are carried out in the same tube without opening between steps. Optimized to deliver highly sensitive quantification of RNA viruses or low abundance RNA targets in uni- or highly multiplexed RNA detection assays, this reagent chemistry is optimized to deliver maximum assay sensitivity, precision and reproducibility with miniaturized reaction volumes and either conventional or accelerated thermal cycling protocols. UltraPlex 1-Step ToughMix contains all required components for RT-qPCR except RNA template and probe and is compatible with all dual-labeled probe chemistrie.
Features & Benefits
- 4X concentrated master mix allows greater flexibility with dilute samples – as little as 1 pg of total RNA
- Multiplexing capable with up to 5 targets
- Tough-tested for applications where PCR inhibition from template impurities may be a concern, such as: crude extracts, clinical specimens, or environmental samples.
- Temperature stabilized master mix enables convenient reaction setup at ambient room temperature
| Product | Kit Size | Order Info |
|---|---|---|
| qScript XLT 1-Step RT-qPCR ToughMix ROX | 100 x 20 μL rxns (1 x 1 mL) | 95133-100 |
| 500 x 20 μL rxns (5 x 1 mL) | 95133-500 | |
| 2000 x 20 μL rxns (1 x 20 mL) | 95133-02K | |
| qScript XLT 1-Step RT-qPCR ToughMix | 100 x 20 μL rxns (1 x 1 mL) | 95132-100 |
| 500 x 20 μL rxns (5 x 1 mL) | 95132-500 | |
| 2000 x 20 μL rxns (1 x 20 mL) | 95132-02K | |
| 5000 x 20 µL rxns (1 x 50 mL) | 95132-05K | |
| qScript XLT 1-Step RT-qPCR ToughMix Low-ROX | 100 x 20 μL rxns (1 x 1 mL) | 95134-100 |
| 500 x 20 μL rxns (5 x 1 mL) | 95134-500 | |
| 2000 x 20 μL rxns (1 x 20 mL) | 95134-02K | |
| 5000 x 20 µL rxns (1 x 50 mL) | 95134-05K |
qScript XLT One-Step RT-qPCR ToughMix is a ready-to-use, highly sensitive master mix for reverse transcription quantitative PCR (RT-qPCR) of RNA templates using hybridization probe detection chemistries such as TaqMan 5′-hydrolysis probes. First-strand cDNA synthesis and subsequent PCR amplification are carried out seamlessly in the same reaction mixture with optimized 1-step thermal cycling parameters.
This kit is ideal for highly sensitive quantification of RNA viruses or low abundance RNA targets as well as high throughput gene-expression studies. The system has been optimized to deliver maximum RT-PCR efficiency, sensitivity, and specificity in minimal reaction volumes and accelerated thermal cycling rates. The only necessary user-supplied materials for RT-qPCR is RNA sample and probe assay. It is compatible with all types of molecular probe assays including dual-labeling strategies.
Elevating cDNA synthesis reaction temperature to 50-55°C during one-step RT-qPCR improves primer annealing and disruption of RNA secondary structure that can interfere with cDNA synthesis.
Features & Benefits
- Tough Tested – Overcomes common inhibitors including polysaccharides, heme/hemoglobin, humic acid, melanin
- Flexible – Use fast or standard qPCR cycling conditions
- Broad dynamic range- Reliable data from your precious samples every time
- Multiplexing enabled – Supports highly sensitive detection for up to four targets
| Product | Kit Size | Order Code |
|---|---|---|
| PerfeCTa qPCR ToughMix | 250 x 20 μL rxns (2 x 1.25 mL) | 95112-250 |
| 1250 x 20 μL rxns (10 x 1.25 mL) | 95112-012 | |
| 5000 x 20 μL rxns (1 x 50 mL) | 95112-05K | |
| PerfeCTa qPCR ToughMix ROX | 1250 x 20 μL rxns (10 x 1.25 mL) | 95113-012 |
| 5000 x 20 μL rxns (1 x 50 mL) | 95113-05K | |
| PerfeCTa qPCR ToughMix Low ROX | 250 x 20 μL rxns (2 x 1.25 mL) | 95114-250 |
| 1250 x 20 μL rxns (10 x 1.25 mL) | 95114-012 | |
| 5000 x 20 μL rxns (1 x 50 mL) | 95114-05K | |
| PerfeCTa qPCR ToughMix UNG | 1250 x 20 μL rxns (10 x 1.25 mL) | 95138-012 |
| 5000 x 20 μL rxns (1 x 50 mL) | 95138-05K | |
| PerfeCTa qPCR ToughMix UNG ROX | 1250 x 20 μL rxns (10 x 1.25 mL) | 95139-012 |
| 5000 x 20 μL rxns (1 x 50 mL) | 95139-05K | |
| PerfeCTa qPCR ToughMix UNG Low ROX | 1250 x 20 μL rxns (10 x 1.25 mL) | 95140-012 |
| 5000 x 20 μL rxns (1 x 50 mL) | 95140-05K |
PerfeCTa qPCR ToughMix is a 2X concentrated ready-to-use reaction cocktail for PCR amplification of DNA templates that relieves several types of PCR inhibition commonly encountered with crude extracts, environmental specimens, plant tissues, animal tissues, and complex food matrices. This robust real-time qPCR reagent provides maximum sensitivity and PCR efficiency with a variety of fluorogenic probe chemistries, including TaqMan® hydrolysis probes. The only user-supplied components are primers, probe(s), and DNA template. Pre-blended with inert AccuVue plate loading dye to help minimize pipette errors during setup and provides visual confirmation of thorough mixing. A key component of PerfeCTa qPCR ToughMix is an ultra pure, highly processive thermostable DNA polymerase that is combined with high avidity monoclonal antibodies. This proprietary polymerase mix is highly resistant to PCR inhibitors and provides an extremely stringent automatic hot-start allowing reaction assembly, and temporary storage, at room temperature prior to PCR amplification. PerfeCTa qPCR ToughMix delivers exceptional performance with either fast or conventional PCR cycling protocols. UNG containing versions are blended with Uracil N-glycosylase to eliminate potential post-PCR carryover contamination associated with routine molecular testing.
Features & Benefits
- ToughMix additives neutralize PCR inhibitors to ensure reliable assay performance with a spectrum of starting materials including: clinical specimens, plants, soil, environmental or complex food matrices
- Superior assay sensitivity and target precision with AccuStart™ II enzyme technology – maximum-yielding, engineered Taq DNA polymerase with stringent, ultrapure antibody hotstart
- Easy-to-use 2X concentrated master mixes with AccuVue™ plate loading dye and optimized passive reference dye for simplified reaction setup
- Supports efficient vortex mixing with proprietary anti-foaming technology
- Consistent and reliable performance with exceptional reagent stability (uninhibited after 20X freeze-thaw or 30 days at 22°C)
| Product | Kit Size | Order Code |
|---|---|---|
| Genotyping ToughMix ROX | 1250 x 20 μL rxns (10 x 1.25 mL) | 95116-012 |
| 5000 x 20 μL rxns (1 x 50 mL) | 95116-05K | |
| Genotyping ToughMix Low ROX | 1250 x 20 μL rxns (10 x 1.25 mL) | 95117-012 |
| 5000 x 20 μL rxns (1 x 50 mL) | 95117-05K |
Genotyping ToughMix is a 1-tube qPCR SuperMix reagent compatible with all dual-label (hydrolysis) probe chemistries for both fast and conventional PCR cycling protocols or instruments. This proprietary formulation has been rigorously optimized to destabilize single base-pair mismatches to ensure precise allelic discrimination and cluster separation with SNP detection assays. The reagent is provided as a 2x concentrated ready-to-use reaction cocktail that contains all required reaction components, except primers, probe(s), and DNA template. Inert AccuVue plate loading dye helps to minimize pipette error and provides visual confirmation of thorough mixing.
Features & Benefits
- Optimized buffer chemistry destabilizes single base-pair mismatch probes, providing superior allelic discrimination and improved cluster separation for critical, single-nucleotide polymorphism (SNP) detection assays
- Sensitive, precise detection with ultrapure AccuStart II enzyme technology – maximum-yielding Taq DNA polymerase mutant controlled by stringent, multi-epitope antibody hotstart
- ToughMix reagent technology neutralizes a broad spectrum of PCR inhibitors common in plant and animal tissues, environmental sources, clinical specimens and complex food matrices
- Easy-to-use 2x concentrated SuperMix with AccuVue plate loading dye and pre-blended passive reference dye simplifies reaction setup
- Supports efficient vortex mixing with proprietary anti-foaming technology
| Product | Kit Size | Order Code |
|---|---|---|
| PerfeCTa SYBR Green FastMix for IQ | 1250 x 20 μL rxns (10 x 1.25 mL) | 95071-012 |
| 5000 x 20 μL rxns (1 x 50 mL) | 95071-05K | |
| PerfeCTa SYBR Green FastMix | 250 x 20 μL rxns (2 x 1.25 mL) | 95072-250 |
| 1250 x 20 μL rxns (10 x 1.25 mL) | 95072-012 | |
| 5000 x 20 μL rxns (1 x 50 mL) | 95072-05K | |
| PerfeCTa SYBR Green FastMix ROX | 250 x 20 μL rxns (2 x 1.25 mL) | 95073-250 |
| 1250 x 20 μL rxns (10 x 1.25 mL) | 95073-012 | |
| 5000 x 20 μL rxns (1 x 50 mL) | 95073-05K | |
| PerfeCTa SYBR Green FastMix Low ROX | 250 x 20 μL rxns (2 x 1.25 mL) | 95074-250 |
| 1250 x 20 μL rxns (10 x 1.25 mL) | 95074-012 | |
| 5000 x 20 μL rxns (1 x 50 mL) | 95074-05K |
PerfeCTa SYBR® Green FastMix is a 2X concentrated, ready-to-use reaction cocktail that contains all components, except primers and DNA template. This rigorously optimized master mix contains of proprietary buffer technology, stabilizers and AccuFast Taq DNA polymerase to deliver maximum assay precision, sensitivity, and PCR efficiency for accelerated or conventional thermal cycling conditions for SYBR Green detection. Dye-based detection methods are critically dependent on highly specific amplification because dsDNA dyes will bind to any amplicon, including off-target primer elongation and primer dimerization. AccuFast hot start Taq DNA polymerase contains a proprietary mixture of ultra-pure monoclonal antibodies that stringently suppress primer elongation prior to the initial PCR denaturation step and allows for setup and multi-day storage at ambient room temperature prior to thermal cycling. AccuFast provides rapid release of fully active enzyme to support accelerated thermal cycling conditions.
Features & Benefits
- 2x concentrated reagents minimize pipetting steps, simplify reaction assembly and improve accuracy
- Superior assay sensitivity and specificity with ultrapure AccuStart enzyme technology – maximum-yielding Taq DNA polymerase mutant controlled by stringent, multi-epitope antibody hot start
- Supports efficient vortex mixing with proprietary anti-foaming formulation
- FastMix formulation supports both fast and standard thermal cycling conditions
| Product | Kit Size | Order Code |
|---|---|---|
| Qscript lyo 1-step | 24 rxns | 95198-024 |
Qscript lyo 1-step is a lyophilized single-reaction reagent optimized for highly sensitive and reproducible one-step RT-qPCR using hydrolysis probes. The reagent contains a hot-start thermo-stable polymerase, a genetically engineered reverse transcriptase as well as other components to ensure higher performance detection of up to 5 targets with maximum sensitivity and specificity. The enhanced stability of the freeze-dried master mix enables convenient shipping and storage at room temperature. The single tube reaction facilitates easy reaction set up while preventing potential cross-contamination.
Features & Benefits
- Lyophilized Single Tube Format : Easy-to-use, reduces cross-contamination
- High Sensitivity & Specificity : Detect as low as 0.5 pg RNA
- Wide Dynamic Range : 0.5 – 500 pg RNA
- Superior Multiplexing : Plex up to 5 targets per reaction
- Eliminate Freezer Storage : Up to 9 months stability room temperature
Brand
Quantabio is a leading provider of advanced DNA and RNA amplification reagents for the most demanding molecular testing applications in applied, translational, and life science research. Our team leverages decades of experience in developing pioneering amplification technologies to deliver cutting-edge products to researchers focused on critical cloning, PCR, qPCR, and Next-Generation Sequencing (NGS) applications. Based in Beverly, Massachusetts, Quantabio offers a growing portfolio of products through its international sales operations, as well as a global network of distributors and commercial service providers.