Pacbio – Short-read Sequencing – Onso System
The Pacbio – Short-read Sequencing – Onso System is an innovative benchtop short-read DNA sequencing platform with an extraordinary level of accuracy using PacBio sequencing by binding (SBB) technology. Ultra-high Q40+ data quality allows you to break through limits of detection.
The Onso short-read sequencing system is a scalable and flexible benchtop platform that provides an extraordinary level of accuracy through its unique sequencing by binding (SBB™) technology. With inherently lower sequencing noise, the Onso system enables researchers to uncover rare variants previously missed and do so at lower sequencing depths.
The accuracy provided by the Onso system will help drive groundbreaking discoveries in areas such as liquid biopsy testing for minimal residual disease (MRD), antibiotic resistance monitoring in microbial communities, off-target analysis in gene editing experiments, and many other “needle-in-a-haystack” applications where the highest level of sensitivity is desired.
Sequencing Specifications
| Onso reagents | Read length | Reads | Output (GB) | Run time | Quality score |
|---|---|---|---|---|---|
| 200 cycle sequencing kit | 2×100 bp 1×200 bp | 400—500 M | 80—100 | 32 hours | ≥90% Q40 |
| 300 cycle sequencing kit | 2×150 bp | 400—500 M | 120—150 | 48 hours | ≥90% Q40 |
Technical Specifications
| Temperature | 18–26°C (64–79°F) |
| Humidity | 20–80% relative humidity, non-condensing |
|---|---|
| Altitude | Below 2,000 m (6,562 ft) |
| Sequencer dimension (W x D x H) | 37 in (94 cm) × 29 in (73.7 cm) × 25.75 in (65.4 cm) |
| Weight | 133.8 kg (295 lb) |
| Cluster generator dimension (W x D x H) | 32 in (81.3 cm) x 22 in (55,9 cm) x 26 in (66 cm) |
| Weight | 43.5 kg (96 lb) |
| Power requirements | 100–240 V at 50–60 Hz |
| Network connection | ≥10 Gbps |
| ICC operating system | Windows 10 Enterprise |
| Output file format | FASTQ |
| Output file size | 35–60 GB |
Key Features
A Groundbreaking New Sequencer
Brand
PacBio offers revolutionary sequencing technologies that combine the completeness of long reads with the accuracy of short reads to provide the most comprehensive view of genomes, transcriptomes, and epigenomes.