Clear 3D Cell Culture Models With Minimal Changes to Morphology and Without Compromising Sensitivity

Despite the growing relevance of 3D cell culture as a research model, imaging techniques used to characterize these models are highly limited. Due to the thickness and opacity of 3D cellular structures, most current imaging technologies cannot penetrate to the center of the tissues, resulting in only the outer 2 to 3 layers of cells being detected. This causes the dark centers often seen in images of 3D cell culture models, which proves highly problematic for accurate analysis as these outer cells are most exposed to compounds, nutrients and oxygen and thus do not reflect the entire cell population.

Corning now offers 3D Clear Tissue Clearing reagent that can be used in a tissue clearing technique, designed specifically to support imaging for 3D cell culture models and plate-based high throughput processing. When paired with fluorescent labeling (e.g., fluorescent protein, immunofluorescence, chemical dyes) and high content confocal microscopy, Corning 3D Clear reagent allows for complete 3D cell culture model characterization and more accurate drug screening.

With L-glutamine Leibovitz’s L15 medium was originally developed without a sodium bicaronate buffer system for use in non-CO2 equilibrated environments.  It is buffered by phosphates and the amino acid L-arginine, along with L-histidine and L-cysteine.  The formation of acid metabolite byproducts is prevented by replacing glucose with galactose and sodium pyruvate.  Examples of usage include the propagation of HEP-2, monkey kidney cells and primary explants of embryonic and adult human tissue.  Leibovitz’s L-15 supports established cell lines as well as primary explants of embryonic and adult human tissue.

Complete Certificate of Analysis available for each production lot, along with full formulation information Manufactured in a facility that complies with ISO 13485 certified quality system. This product is produced under high quality industry standards

Custom Media and Serum from Corning

From cell culture and antibody production to vaccine development and drug discovery, our high-quality custom media development and manufacturing services can produce customized, tailored media and reagents to meet your unique research needs.

Custom media products are manufactured under the current ISO 13485 standard and FDA Quality System Regulation 21 CRF 820, current good manufacturing practices (cGMP), FDA-registered facility for Class 1 Medical Devices, we offer industry-leading media products and process quality that meets all cGMP and FDA requirements for manufacturing and sterility.

Custom media and serum from Corning, can provide you with a full range of capabilities to meet your custom media formulation, packaging, and regulatory requirements and support you with experience and expertise along the way.

Growth Promotion and Cytotoxicity Corning classical media products undergo a variety of tests to determine functionality and toxicity using growth-promotion analysis. Product performance is measured by harvest-to-plant ratios or fold increase according to predetermined specifications.

Physiochemical Properties Liquid and powder classical media products are tested to determine pH and osmolality. Classical media powders are also tested for residual moisture. The pH and osmolality tests are performed according to specific procedures in which all equipment is calibrated using standards traceable to the National Institute of Standards and Technology.

Biological Tests Quality release testing of Corning liquid classical media products assures low levels of endotoxin, mycoplasma, and an absence of contamination.

Endotoxin Classical media products are tested for bacterial endotoxin using the Limulus Amoebocyte Lysate (LAL) chromogenic assay. For release, each lot of standard liquid classical media must have an endotoxin level of <0.25 EU/mL. Classical media powder products must have an endotoxin level of <0.25 EU/mL when reconstituted to formula weight with low endotoxin water.

Sterility All Corning liquid classical media products are tested for sterility.

Mycoplasma Liquid classical media products are tested for mycoplasma using a large-volume method.

This proprietary formulation is not available from other suppliers Complete Certificate of Analysis available for each production lot, along with full formulation information Manufactured in a facility that complies with ISO 13485 certified quality system. This product is produced under high quality industry standards

With Corning® glutagro™ SupplementCorning hybrigro SF is a complete, animal component-free, defined medium specifically developed for serum-free growth and antibody production with a variety of hybridoma cell lines. -Superior growth and antibody production over serum supplemented media. -Formulated with glutagro to provide a stable, ready to use formulation. No glutamine supplementation required. -Contains Polaxamer 188 to provide shear force protection during agitated suspension culture. -Formulated without phenol red. -Contains low concentrations of highly purified non-animal origin recombinant proteins added for enhanced cell growth and productivity. -Defined: There are no undefined hydrolysates, ultra filtered lysates, or peptides in the formulation.

Complete Certificate of Analysis available for each production lot, along with full formulation information Manufactured in a facility that complies with ISO 13485 certified quality system. This product is produced under high quality industry standards.

Ideal for the preparation of reagents, rinsing glassware or plasticware, and other molecular biology or cell culture applications, Corning® water can save the expense of installing and maintaining your own water system, while ensuring you have a secondary source of water that meets your specifications.

Corning water is available in a variety of sizes, and tested to specifications which may include USP and/or EP monograph requirements.

Beaker, with spout, manufactured with uniform wall thickness, offers optimum balance between thermal shock resistance and mechanical strength. For convenience, the 250 through 4000 mL beakers have a double graduated metric scale to indicate approximate content. All sizes have an extra large marking spot. The 10 mL size is not graduated.

Desiccators have a rugged 24/29 standard taper stopcock with a tooled hose connection that serves as the vacuum inlet. It is designed so that its manipulation is not affected by the vacuum.

Calibrated “To Contain.” A less costly cylinder, designed for those institutions performing many general laboratory procedures. No bumper guards supplied.

Capacity:10 mL, 25 mL, 50 mL, 100 mL, 250 mL, 500 mL, 1 L, 2 L

 

PYREX® flat bottom boiling flasks have long necks with tooled mouths and take a No. 2 rubber stopper. They are manufactured from No. 7740 borosilicate glass. Wall thicknesses are carefully controlled to provide the proper balance between thermal expansion and mechanical strength for greater shock resistance. Their excellent chemical durability results in contamination-free results and long life under the toughest lab conditions. The normal working temperature for PYREX® boiling flasks is 230°C.

These flasks, with tubulation, are blown in special molds, in shapes designed to give maximum mechanical strength. For convenience, the flasks are graduated to show approximate capacity. The neck finish affords an excellent fit for rubber stoppers. Tubulation O.D. on sizes up to 1000 mL is approximately 10 mm. Tubulation O.D. for 2000 mL, and 4000 mL is approximately 12.5 mm.

Approximate graduations are in durable white enamel. An extra large marking space is also provided. Heavy, durable top tooling.

A convenience pack containing one each of the most popular sizes of Cat. No. 4980 series flask. Designed for the low volume user, a case contains one each of five sizes: 50 mL, 125 mL, 250 mL, 500 mL, and 1L. Packaged in a partitioned carton for safe transit and storage.