Corning – Media – DMEM (Dulbecco’s Modified Eagle’s Medium)
Dulbecco’s Modified Eagle Medium (DMEM) is modified to contain four times the amino acid and vitamin concentration of original Eagle’s Minimal Essential Medium. The original DMEM formulation contained a low concentration of …
Dulbecco’s Modified Eagle Medium (DMEM) is modified to contain four times the amino acid and vitamin concentration of original Eagle’s Minimal Essential Medium. The original DMEM formulation contained a low concentration of glucose (1 g/L) and sodium pyruvate. However, DMEM is now available in a variety of formulations, including those with higher glucose levels and with or without sodium pyruvate. DMEM also uses a sodium bicarbonate buffer system, allowing for maintenance of physiological pH in a 5–10% CO2 environment. Importantly, DMEM commonly requires fetal bovine serum (FBS) supplementation.